Optimisation of pulsed electric fields extraction of anthocyanin from Beibinghong Vitis Amurensis Rupr |
| |
| Authors: | Yang He Jingsheng Liu Yueru Li Fei Zheng Weihong Min |
| |
| Institution: | 1. Department of Food Science and Engineering, Jilin Agricultural University, Changchun, China;2. Agricultural Quality Standards and Testing Technology Research Center, Changchun, China;3. Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China |
| |
| Abstract: | Beibinghong Vitis amurensis Rupr has wide plantation area, high productivity and rich anthocyanin. Common hot-extraction has poor deficiency and destroys anthocyanin severely. For Beibinghong V. amurensis Rupr as materials, response surface-optimised electric fields were used, the structure of Beibinghong was observed by SEM, antioxidant activity was measured by DPPH, ABTS and reducing force, the component of anthocyanin was analyzed by HPLC-MS. We found the content of total anthocyanin extracted by pulsed electric fields was 166.65 ± 3.88 mg/100 g.FW. Total anthocyanin from Beibinghong had high antioxidant activity, also contained multiple steady anthocyanin of delphinidin 3-O-glucoside, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, delphinidin-3-O-(6-O-acetyl) glucoside and delphinidin-3-O-(6-O-p-coumaroyl) glucoside et al. In conclusion, the optimised pulsed electric fields method can quickly and efficiently extract several kinds of anthocyanins from V. amurensis Rupr. This study promoted the intensive processing of V. amurensis Rupr and widened the practical application of pulsed electric field technology. |
| |
| Keywords: | Beibinghong anthocyanin pulsed electric fields SEM HPLC-MS |
|
|
