Protein type effects on steady-state crossflow membrane ultrafiltration fluxes and protein transmission |
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Authors: | Gerda Grund Campbell W Robinson and Bernard R Glick |
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Institution: | a Department of Chemical Engineering, University of Waterloo, Waterloo, Ont. N2L 3G1 Canada b Department of Biology, University of Waterloo, Waterloo, Ont. N2L 3G1 Canada |
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Abstract: | The permeate fluxes and percent protein transmission were evaluated for steady-state crossflow ultrafiltration of two proteins of different composition: bovine serum albumin (BSA), containing fatty acid, and “fatty-acid-poor” BSA, from which most of the fatty acids had been removed (BSA/FAP). The influences of protein concentration up to 6.5 percent w/v, transmembrane pressure, ionic environment and membrane type (i.e. nominal molecular weight cut-off) were investigated. For both BSA and BSA/FAP, the fluxes and the protein transmission were dependent on the amount of salt present. The higher fatty acid content in the BSA apparently enhanced protein-protein interaction, resulting in a more cohesive and resistant fouling layer; permeate fluxes were lower with BSA/FAP than with BSA at otherwise corresponding operating conditions. A hysteresis behaviour of the flux (J)-transmembrane pressure (TMP) relationship was observed whenever the ultrafiltration unit was operated at a TMP less than some higher value to which the membrane previously had been exposed. |
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Keywords: | ultrafiltration permeate flux resistances microporous membranes protein concentration bovine serum albumin |
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