| 两种乳糖酶活力测定方法比较 |
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| 引用本文： | 何云山,刘娅薇,谭周进,惠华英.两种乳糖酶活力测定方法比较[J].应用化学,2020,37(2):242-244. |
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| 作者姓名： | 何云山 刘娅薇 谭周进 惠华英 |
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| 作者单位： | 药学院 湖南中医药大学 长沙 410208;医学院 湖南中医药大学 长沙 410208 |
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| 基金项目： | 国家自然科学基金青年基金(81703985)项目资助 |
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| 摘 要： | 邻硝基苯-β-D-吡喃半乳糖苷(ONPG)法和双糖酶法是常用的乳糖酶活力测定方法,本文对这两种方法的线性范围、检出限、精密度、准确度和稳定性进行了比较。 结果显示,ONPG法在线性范围、检出限、精密度以及稳定性方面均优于双糖酶法,但此种方法的重复性较差,需要进一步的优化。 对比这两种方法,可为乳糖酶活力测定方法的合理选取提供参考。
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| 关 键 词： | 邻硝基苯-β-D-吡喃半乳糖苷法 双糖酶法 乳糖酶活力测定 方法学比较 |
| 收稿时间： | 2019-08-03 |
Comparison of Two Lactase Activity Determination Methods |
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| HE Yunshan,LIU Yawei,TAN Zhoujin,HUI Huaying.Comparison of Two Lactase Activity Determination Methods[J].Chinese Journal of Applied Chemistry,2020,37(2):242-244. |
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| Authors: | HE Yunshan LIU Yawei TAN Zhoujin HUI Huaying |
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| Institution: | College of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;School of Medicine,Hunan University of Chinese Medicine,Changsha 410208,China |
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| Abstract: | Both o-nitrophenyl β-D-galactopyranoside(ONPG) method and disaccharase method are commonly used for the determination of lactase activity. This paper compared the linear range, detection limit, precision, accuracy and stability of these two methods. It is found that ONPG method is better than disaccharase method in linear range, detection limit, precision and stability, but this method has poor repeatability and needs further optimization. |
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| Keywords: | o-nitrophenyl-β-D-galactopyranoside disaccharase method lactase activity methodological comparison |
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