Imaging of bacterial infection with 99mTc-labeled HBD-1 |
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| Authors: | F Yurt Lambrecht O Yilmaz P Unak B Seyitoglu K Durkan S Yolcular H Baskin |
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| Institution: | (1) Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey;(2) Department of Animal Research Center, Dokuz Eylul University School of Medicine, Izmir, Turkey;(3) Department of Microbiology, Dokuz Eylul University School of Medicine, Izmir, Turkey |
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| Abstract: | The aim of this study was to evaluate 99mTc labeled human β-defensin-1 (HBD-1) for discrimination between bacterial infection and sterile inflammation. For this purpose,
HBD-1 was radiolabeled with 99mTc and its in vivo distribution was evaluated in inflamed rats with Staphylococcus aureus (S. aureus) and sterile inflamed rats with turpentine oil. After injection into inflamed and sterile inflamed rats, 99mTc-HBD-1 was rapidly removed from the circulation via the kidneys. Binding of 99mTc-HBD-1 to inflamed muscle (T/NT = 20 at 120 min) was two times higher than binding to sterile inflamed muscle (T/NT = 10
at 120 min) of rats. It was demonstrated that 99mTc-HBD-1 can be used to detect S. aureus inflammation in rats. However, the radiolabeled antimicrobial peptide showed only poor uptake in sterile inflammation with
turpentine oil in rats. As a result, 99mTc-HBD-1 can be useful for detection of bacterial inflammation. |
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