Lateral mobility of plasma membrane proteins in dividing eggs of the loach (Misgurnus fossilis): Regional differences and changes during the cell cycle |
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Authors: | Valentina P Bozhkova Monika Budayova Peter Kvasnicka Natalia Cigankova Dusan Chorvat |
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Institution: | 1. Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia 2. Department of Biophysics and Chemical Physics, Faculty of Mathematics and Physics, Comenius University, Bratislava, Slovakia
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Abstract: | Regional differences in lateral diffusion rates of fluorescence-labeled proteins have been studied in the plasma membrane of dividing eggs of the loach (Misgurnus fossilis) by fluorescence recovery after photobleaching (FRAP). Apparent animal-vegetal differences in fluorescence intensity, lateral diffusion coefficients, and fractions of mobile proteins have been found, with all these quantities being higher in the animal pole region than in the yolk region. Cyclic changes in protein diffusion coefficients and mobile fractions during the first few cell cycles have also been recorded. Soon after the end of a cleavage, the diffusion coefficient reaches its minimal value and increases rapidly before the next cleavage. |
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Keywords: | Polarity cell cycle plasma membrane translational mobility photobleaching |
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