Bioactive Polymers. XXVI. Immobilization of Pepsin on Biozan R |
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| Authors: | Cristofor Simionescu Severian Dumitriu Marcel Popa Cristina Coman Vlad Artenie |
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| Institution: | 1. Department of Organic and Macromolecular Chemistry , Polytechnic Institute of Jassy , 6600, Jassy, Romania;2. “A. I. Cuza” University , 6600, Jassy, Romania |
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| Abstract: | Pepsin was immobilized on BIOZAN R (Hercules) with dicyclohexylcarbodiimide as activator. The reaction product obtained has a protein content of 35–110 mg/g of polymer and a proteolytic activity between 20.85–28.75 μmol tyrosine/L·min·g of polymer). The coupling reaction rate is maximum under the following conditions: pepsin/BIOZAN R ratio = 0.52 g/g, DCCI/BIOZAN R ratio = 0.2 g/g, pH = 3.4, reaction time = 4 h. |
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| Keywords: | Iodonium salts Photosensitization Thioxanthone Potolysis Potoinitiated cationic polymerization |
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