Use of packed-fiber solid-phase extraction for sample clean-up and preconcentration of vitamin B_(12) before determination |
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Authors: | Fang Fanga Xue Jun Kang Zhi Yong Liua Yu Qin Maa Zhong Ze Gub a Key Laboratory of Child Development Learning Science School of Public Health Southeast University Nanjing China b State Key Laboratory of Bioelectronics Nanjing China |
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Institution: | a Key Laboratory of Child Development and Learning Science, School of Public Health, Southeast University, Nanjing 210009, China;b State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China |
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Abstract: | A rapid and simple preconcentration step applying packed-fiber solid-phase extraction columns has been investigated to vitamin B12. The extraction performance of the new method was investigated preliminarily on vitamin functional drink. The analysis used a reversed-phase C18 column, with a photo-diode array detector at 220 nm. The samples were preconcentrated with packed-fiber solid-phase extraction columns. Good linearity was observed in vitamin functional drink. The repeatability of extraction performance, expressed as relative standard deviations, was from 3.5% to 4.3%. The limit of detection (LOD) is 5 ng mL−1 (S/N = 3). Finally, the method had been applied for the determination of vitamin B12 in vitamin functional drink. |
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Keywords: | Vitamin B12 Nanofibers Packed-fiber solid-phase extraction Preconcentration |
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