铜络合物与生物小分子次黄嘌呤相互作用的光谱电化学和荧光光谱

以光谱电化学和荧光光谱法证明了络合物Ｃｕ（ｐｈｅｎ）２２＋和Ｃｕ（ｂｐｙ）２２＋与次黄嘌呤能发生络合作用并求出它们的络合比为 １．络合常数分别为 １． ２１× １０４ Ｌ／ｍｏｌ和 ２． ３２ × １０４Ｌ／ｍｏｌ；相互作用的产物的光谱电化学性质发生了明显的变化，结合了次黄嘌呤后的 Ｃｕ（Ｐｈｅｎ）２＋－ＨＸ和Ｃｕ（ｂｐｙ）２＋－ＨＸ在空气中氧化的动力学常数明显减小．这些相互作用的定量数据为铜络合物与ＤＮＡ作用部位提供了重要信息．

Study on Interaction of Cu (phen )_2~(2+) and Cu (bpy )_2~(2+) With Hypoxanthine by Means of the Spectroelectrochemistry and Fluorescence Spectrometry

In this paper, it has been proved that complexes of Cu (phen)22+ and Cu (bpy)22+ can interact with hypoxanthine (HX). The spectroelectrochemical and fluorescence behaviors of the copper complexes change obviously in the presence of hypoxanthine. The coordination constants of Cu (phen)22+ and Cu (bpy)22+ with hypoxanthine were determined to be 1. 21 X 104 L/mol and 2. 32X 104 L/mol, respectively,and the coordination ratios of them are 1. The Spectroelectrochemical experiments revealed that both Cu (phen )+2-HX and Cu (bpy)2+-HX can also be oxidized by O2 and comparing with those in the absence of hypoxanthine, their kinetic constants are reduced about 40%. These data of the interactions of Cucomplexes with hypoxanthine can provide a useful information for the further study on the interaction of copper complexes with deoxyribonucleic acid.