| 柱前衍生高效液相色谱法对动物组织中胶原蛋白的测定 |
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| 引用本文： | 郑家概,王飞,农云军,闫世平,李光宪.柱前衍生高效液相色谱法对动物组织中胶原蛋白的测定[J].分析测试学报,2008,27(12). |
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| 作者姓名： | 郑家概 王飞 农云军 闫世平 李光宪 |
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| 作者单位： | 中国广州分析测试中心,广东省化学危害应急检测技术重点实验室,广东,广州,510070 |
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| 基金项目： | 广东省自然科学基金资助项目(7300055) |
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| 摘 要： | 动物组织中的胶原蛋白经酸水解后生成包括羟脯氨酸在内的氨基酸混合物,用邻苯二甲醛（OPA）与其中的一级氨基酸衍生,再用9-芴基甲氧基羰酰氯（FMOC）与其中的羟脯氨酸衍生,用反相高效液相色谱法测定羟脯氨酸含量。羟脯氨酸是胶原蛋白的特异性氨基酸且含量稳定,因而可通过样品中羟脯氨酸的含量计算胶原蛋白含量。在0.01-50 mg.L^-1范围内,羟脯氨酸的峰面积和质量浓度之间的相关系数为0.9993,保留时间和峰面积的相对标准偏差分别为0.30%和2.9%。该法选用FMOC与氨基酸衍生产物的特征波长检测,能够完全屏蔽一级氨基酸衍生物的干扰,可快速、准确、高效地测定羟脯氨酸及胶原蛋白的含量。
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| 关 键 词： | 柱前衍生 高效液相色谱法 胶原蛋白 羟脯氨酸 |
Determination of Collagen in Animal Tissues by Pre-column Derivatization and High Performance Liquid Chromatography |
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| ZHENG Jia-gai,WANG Fei,NONG Yun-jun,YAN Shi-ping,LI Guang-xian.Determination of Collagen in Animal Tissues by Pre-column Derivatization and High Performance Liquid Chromatography[J].Journal of Instrumental Analysis,2008,27(12). |
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| Authors: | ZHENG Jia-gai WANG Fei NONG Yun-jun YAN Shi-ping LI Guang-xian |
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| Abstract: | After hydrolysis with hydrochloric acid,the collagen in animal tissues was decomposed into multiform amino acids mixture,including hydroxyproline.Then the primary amino acids and hydroxyproline were derived with OPA and FMOC,respectively.The content of hydroxyproline was determined by reversed-phase high performance liquid chromatography(RP-HPLC).Because hydroxyproline was the specific amino acid of collagen and the content was stable,the content of collagen could be calculated by that of hydroxyproline in ... |
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| Keywords: | pre-column derivatization HPLC collagen hydroxyproline |
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