Scope,limitations and mechanism of nucleotide separation by thin-layer chromatography with strong cation-exchange resin containing layers

Summary The scope and limitations of the application of the commercially available cation-exchange thin-layer chromatoplate (Fixion 50-X8) for the resolution of nucleotide mixtures is presented. A possible separation mechanism is proposed and discussed.Publication No. 11 in the series dealing with the separation of nucleic acid bases, nucleosides and nucleotides on thin-layers consisting of strong cation-exchange resins.