Correlation between CMC and chromatographic index: simple and effective evaluation of the hydrophobic/hydrophilic balance of bile acids |
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Authors: | Benedetto Natalini Roccaldo Sardella Emidio Camaioni Antimo Gioiello Roberto Pellicciari |
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Institution: | (1) Dipartimento di Chimica e Tecnologia del Farmaco, Università degli Studi di Perugia, Via del Liceo, 1, 06123 Perugia, Italy |
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Abstract: | The discovery that bile acids are involved in the modulation of nuclear steroid receptors has prompted renewed interest in
this field of research. Due to the nature of research in this field, a technique that enables simple and effective assessment
of the hydrophobic/hydrophilic balance, thus improving and speeding up evaluations of the biological profiles of these compounds,
is greatly needed. In this context, both CMC value determination and RP-HPLC mobility evaluation were explored as possible
approaches. While the CMC was calculated using the noninvasive conductimetric method, the RP-HPLC mobility was assessed by
measuring the retention factor at several mobile phase compositions and extrapolating back to the pure aqueous mobile phase.
The correlation of the CMC with the derived chromatographic hydrophobic index ϕ
0 was satisfactory.
Figure Experimental versus predicted pCMC values |
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Keywords: | Critical micellar concentration Hydrophobicity index Conductimetry Noninvasive method |
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