Partial transesterification of sunflower oil with ethanol by a silica fiber reinforced aerogel encapsulated lipase |
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Authors: | Ali Karout Alain C Pierre |
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Institution: | 1.CNRS UMR 5256, IRCELYON, Institut de recherches sur la catalyse et l’environnement de Lyon,Université Lyon 1,Villeurbanne,France |
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Abstract: | A commercial lyophilized lipase of Burkholderia cepacia, from Amano, was encapsulated in silica aerogels reinforced with silica quartz fiber felt. This biocatalyst was applied in
the direct transesterification of sunflower seed oil with ethanol, without any other solvent. When the molar ratio of ethanol
to oil was two or three, the oil transformation kinetics was found to be very slow after the formation of 1 mole of fatty
ethyl ester per mole of initial triglyceride. For a molar proportion of ethanol to oil ≈1, the recycling activity also decreased
gradually in successive tests to reach an activity ≈7% of the initial activity, during the 5th test. Textural and structural
analysis of the aerogels before and after catalytic tests showed that this deterioration was associated with a modification
of the aerogel, by preferential adsorption of glycerol or possibly other transesterification products such as diglycerides.
Besides, it is proposed that one of the cause for the aerogel loss of activity at an initial molar ratio of ethanol:oil of
3:1 was due to a progressive inhibition of the enzyme by excess adsorbed ethanol. The aerogel samples were also compared to
a commercial product of lipase immobilized on polymer beads, from Fluka. The silica aerogels somewhat improved, to a limited
extent, the activity during recycling. |
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