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New lysine-acetylated proteins screened by immunoaffinity and liquid chromatography-mass spectrometry |
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| Authors: | ZHANG Bing ? ZHAO Chao? LUO KaiXuan YAN GuoQuan YAO Jun WANG YingYin LU HaoJie FAN HuiZhi & YANG PengYuan |
| Affiliation: | ZHANG Bing1,2?,ZHAO Chao2?,LUO KaiXuan2,YAN GuoQuan2,YAO Jun2,WANG YingYin2,LU HaoJie1,2,FAN HuiZhi1,2 & YANG PengYuan1,2 1Department of Chemistry,Fudan University,Shanghai 200433,China,2Institutes of Biomedical Sciences,Shanghai 200032 |
| Abstract: | The lack of selective extraction specific for lysine-acetylated proteins has been a major problem in the field of acetylation biology,though acetylation plays a key role in many biological processes.In this paper,we report for the first time the proteomic screening of lysine-acetylated proteins from a mouse liver tissue,by a new approach of immunoaffinity purification of lysine-acetylated peptides combined with nano-HPLC/MS/MS analysis.We have found 20 lysine-acetylated proteins with 21 lysine-acetylated si... |
| Keywords: | lysine acetylation immunoaffinity purification liquid chromatography mass spectrometry |
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