Lignin biotransformations by an aromatic aldehyde oxidase produced byStreptomyces viridosporus T7A |
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Authors: | Deobald Lee A Crawford Don L |
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Institution: | 1.Department of Bacteriology and Biochemistry, University of Idaho, 83843, Moscow, ID ; |
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Abstract: | Streptomyces viridosporus produces an intracellular aromatic aldehyde oxidase that oxidizes aromatic and α, β-unsaturated aromatic aldehydes to their
corresponding acids. It also produces extracellular oxidase as shown by zones of clearing when grown on agar containing insoluble
dehydrodivanillin (DHDV). This extracellular form may be responsible for oxidizing aldehyde groups in lignin. The extracellular
oxidase was expressed maximally after 3 d growth in medium containing only yeast extract. However, higher levels were produced
when lignocellulose was in the medium. The enzyme was partially purified and its molecular weight was approximated to be about
80,000 daltons. Mutant cultures that had lost the ability to produce zones of clearing on DHDV-containing agar solubilized
smaller quantities of lignin as compared to the wild type, except for one strain. A partially purified oxidase preparation
was shown to oxidize a natural lignocellulose substrate. |
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Keywords: | Index Entries" target="_blank">Index Entries Streptomyces aldehyde oxidase lignin biotransformation degradation |
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