Determination of 16 insect growth regulators in edible Chinese traditional herbs by liquid chromatography electrospray tandem mass spectrometry |
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Authors: | Qian Mingrong Wu Liqin Zhang Hu Xu Mingfei Li Rui Wang Xiangyun Sun Caixia |
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Institution: | (1) State Key Lab Breeding Base for Zhejiang Sustainable Plant Pest Control, MOA Key Lab for Pesticide Residue Detection, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China |
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Abstract: | A new sensitive multiresidue liquid chromatography–tandem mass spectrometry (LC-MS/MS) analytical method for the determination
of 16 insect growth regulator (IGR) residues—RH-5849 (1,2-dibenzoyl-1-tert-butylhydrazine), halofenozide, methoxyfenozide, chromafenozide, fufenozide, tebufenozide, diflubenzuron, chlorbenzuron, triflumuron,
hexaflumuron, novaluron, lufenuron, teflubenzuron, flucycloxuron, flufenoxuron, and chlorfluazuron—in herbs (Perilla frutescens, flos chrysanthemi, lily bulbs, and ginger) has been developed. After the herbs had been extracted with acetonitrile, a combined
graphitized nonporous carbon/aminopropyl (ENVI-Carb/LC-NH2) cartridge and a Florisil cartridge were used to clean up the extracts. LC-MS/MS was performed in multiple reaction monitoring
mode with two specific precursor ion–product ion transitions per IGR to confirm and quantitate the residues in herbs. Quantitation
was performed on the basis of matrix-matched calibrations. The method showed excellent linearity (r
2 > 0.99) and precision (relative standard deviations of 13.6 or lower) for all the target insecticides. The limits of quantitation
were 0.6-10 μg kg-1 for the 16 insecticides in the four herbs. The average recoveries, measured at three concentrations (0.01, 0.1, 1 mg kg-1), were in the range 74.8-105.3%. The method was satisfactorily applied for the analysis of 60 herb samples (Perilla frutescens, flos chrysanthemi, lily bulbs, and ginger). Hexaflumuron was detected at concentrations of 0.029 and 0.051 mg kg-1 in Perilla frutescens. |
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