海葵毒素多肽的分离和初步表征

经冻融法提取,丙酮沉淀,SPSephadexC-25强离子交换和HPLCODS反相色谱层析,从海葵(Ac-tiniacari)中提纯到2个新的毒素多肽,命名为RSAPⅢ和RSAPⅣ。经SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和基质辅助激光解吸电离飞行时间质谱(MAI-DITOP-MS)鉴定,RSAPⅢ和RSAPⅣ均为单一成分,分子量分别为5327和5310,初步钠通道药理学实验表明,RSAPⅢ对豚鼠心室肌细胞钠通道表现出活性,使钠离子内流减少,起阻滞作用。

Isolation and Characterization of Toxin Polypeptides from Sea Anemone Actinia cari

Two toxin polypeptides desgnated RSAPⅢ and RSAP Ⅳ were isolated from sea anemone Ac-tinia cari.Toxins isolation was accomplished by freezing-thawing extraction, fractional acetone precipita-tion, cation exchange chromatography on SP Sephadex C-25 and reverse phase HPLC on ODS C₁₈. Theywere found to be homogenous by SDS-PAGE, and their moleculax weight at m/z 5327 and m/z 5310 re-spectively were determined by MALDI-TOF-MS.RSAP Ⅲ possessed a bioactivity on sodinm channel inGuinea pig cardiac cell to inhibit inward Na⁺ currents, which is contrary to that of all sea anemone toxinsisolated so far.