Analysis of sialyl oligosaccharides by high-performance liquid chromatography-electrospray ionisation-mass spectrometry with differentiation of alpha2-3 and alpha2-6 sialyl linkages |
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| Authors: | Pan Geoffrey Ge Melton Laurence D |
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| Institution: | Food Science Programmes, Department of Chemistry, The University of Auckland, 23 Symonds Rd., Down Town Auckland, Private Bag 92019, Auckland, New Zealand. |
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| Abstract: | A method for analysing sialyl oligosaccharides from bovine colostrum using high-performance liquid chromatography-electrospray ionisation-mass spectrometry (HPLC-ESI-MS) is described. Under positive ionisation mode, mass spectra of alpha2-3 and alpha2-6 linkages were different, and the former produced a prominent B2 (or B3 in disialyl lactose) mass fragment. This fragment was absent from mass spectra with alpha2-6 linkages. Two sialyl oligosaccharides, which have not been reported previously, were tentatively identified. One comprises a N-acetyl neuraminic acid (Neu5Ac), two hexoses (Hex), and one N-acetyl hexosamine (HexNAc) residue ((Neu5Ac)1 (Hex)2 (HexNAc)1), and the other comprises one Neu5Ac and one Hex residue ((Neu5Ac)1(Hex)1). |
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| Keywords: | Sialyl oligosaccharides Bovine colostrum HPLC-ESI-MS Aminopropyl column Sialyl linkage Mass spectrum |
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