溶剂诱导黑色素瘤抗原基因-2特定表位多肽异构的LC/MS研究

 研究乙醇、甲醇两种常用溶剂系统对固相合成的黑色素瘤抗原基因 2 (MAGE 2 )的特定表位多肽 (171 179)异构的影响。分别运用乙醇、甲醇溶剂体系以及极性溶剂二甲基亚砜 (DMSO)作对照预处理固相合成的黑色素瘤抗原特异性MAGE 2表位多肽 (171～ 179) ,然后用反相高效液相色谱 /质谱联用技术 (RP HPLC/MS)对合成的表位多肽的m/z进行Q1正离子监测扫描分析 ,观察其在不同溶剂系统预处理下的异构情况 ,以选择合理的预处理条件。结果发现采用乙醇及甲醇溶样时MAGE 2表位多肽有异构体产生 ,极性溶剂DMSO溶样的表位多肽未有异构现象发生。

LC/MS Research on the Isomers of Special Melanoma Antigen-Encoding Gene-2 Epitope Peptide Induced by Solvent

To study the influence of different solvent systems on the isomers of melanoma antigen encoding gene 2 (MAGE 2) epitope peptide synthesized by Marrifield's solid synthesis method, MAGE 2(171 179) epitope peptides were pre treated using ethanol and methanol systems respectively, and then analysed by RP HPLC/MS, with dimethyl sulphoxide (DMSO) as control solvent Results demonstrated that 100% ethanol and 100% methanol solvents could induce the production of isomers of MAGE 2, while 100% DMSO, 50% ethanol and 50% methanol could not These isomeric peptides could be reversed greatly if treated with trifluoroacetic acid (TFA) The results also showed that the analysis would be distorted and the yield of peptides would decrease dramatically if the peptides were pre treated with high concentrations of ethanol and methanol before RP HPLC analysis and preparation Thus it is suggested that it might be better to decrease the concentration of organic phase in the solvent system for the resolvable hydrophobic peptides, acidified ethanol and methanol might also be better for the highly hydrophobic peptides, and DMSO would be a good solvent for hydrophobic peptides analysis provided that it would not influence the resolving power of chromatography