| Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse.phase High Performance Liquid Chromatography Under Salt and Alkali Stress |
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| 引用本文： | YAN Hong ZHAO Wei JIAO Xin-qian YAN Bing-jun ZHOU Dao-wei. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse.phase High Performance Liquid Chromatography Under Salt and Alkali Stress[J]. 高等学校化学研究, 2006, 22(3): 315-318. DOI: 10.1016/S1005-9040(06)60106-2 |
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| 作者姓名： | YAN Hong ZHAO Wei JIAO Xin-qian YAN Bing-jun ZHOU Dao-wei |
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| 作者单位： | YAN Hong 1，ZHAO Wei 2，JIAO Xin-qian 3，YAN Bing-jun 1 and ZHOU Dao-wei 11. School of Life Sciences，Northeast Normal University，Changchun 130024，P.R. China;2. Department of Avigation Lifesaving，Avigation University of Air Force，Changchun 130022，P. R. China;3. College of Environment and Resources，Jilin University，Changchun 130012，P. R. China |
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| 基金项目： | Acknowledgements We wish to thank Prof. BAI Yu-guang（ Department of Plant Sciences, University of Saskatchewan） for his help in editing this article. We are very grateful to Huang Guo-hui（ School of Life Sciences, Northeast Norreal University） for his help with the RE-HPLC experiments. |
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| 摘 要： | Introduction K.ScopariaisanannualintheChenopodiaceae family.Itsseedscontainabout15%oilandhavebeen usedintraditionalChinesemedicinetotreatdiuresis andskindisease[1].Thecrudeproteincontentof K.Scopariaseedlingsistwiceasmuchasthatin maize.Thisplantisalsorich…
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| 关 键 词： | 有机酸 植物 根部 反向高效液体色谱分析 盐协迫 碱协迫 中药 药物提取 |
| 文章编号： | 1005-9040（2006）-03-315-04 |
| 收稿时间： | 2005-10-10 |
Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phase High Performance Liquid Chromatography Under Salt and Alkali Stress |
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| Hong YAN, Wei ZHAO, Xin-qian JIAO, Bing-jun YAN,Dao-wei ZHOU,. Analysis of Organic Acids Accumulated in Kochia Scoparia Shoots and Roots by Reverse-phase High Performance Liquid Chromatography Under Salt and Alkali Stress[J]. Chemical Research in Chinese University, 2006, 22(3): 315-318. DOI: 10.1016/S1005-9040(06)60106-2 |
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| Authors: | Hong YAN Wei ZHAO Xin-qian JIAO Bing-jun YAN Dao-wei ZHOU |
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| Affiliation: | aSchool of Life Sciences, Northeast Normal University, Changchun 130024, P. R. China;bDepartment of Avigation Lifesaving, Avigation University of Air Force, Changchun 130022, P. R. China;cCollege of Environment and Resources, Jilin University, Changchun 130012, P. R. China |
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| Abstract: | Several organic acids accumulated in Kochia Scoparia shoots and roots were studied by means of reverse-phase high performance liquid chromatography with a C_ 18 column. Five types of binary organic acids were separated. The organic acid concentrations were determined in K. Scoparia seedlings stressed by saline(NaCl) and alkaline (NaHCO_ 3) at the same Na concentration. Concentrations of organic acids are stimulated by alkaline because the cells will adjust their pH values through the accumulation of organic acids, when the environment is basic. The concentrations of oxalic acid and succinic acid are higher than those of other organic acids, including tartaric acid and malic acid, and the concentration of citric acid is the lowest. The concentrations of the organic acids in the roots are higher than those in the shoots under salt(NaCl) stress, but the results are opposite while the roots are under alkali(NaHCO_ 3) stress. This indicates that there are different adaptive strategies for K. Scoparia seedlings in organic acid metabolism under salt and alkali stress. |
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| Keywords: | Kochia Scoparia(L.) Schrad Salt stress Alkaline stress Organic acid HPLC |
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