| 灵敏的单一溴酚蓝测定尿蛋白 |
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| 引用本文： | 方芳,戚玉海,张锋,贺媛媛,赵长容,曹建明. 灵敏的单一溴酚蓝测定尿蛋白[J]. 广东微量元素科学, 2007, 14(6): 37-39 |
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| 作者姓名： | 方芳 戚玉海 张锋 贺媛媛 赵长容 曹建明 |
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| 作者单位： | 温州医学院环境与公共卫生学院,浙江,温州,325035;温州医学院环境与公共卫生学院,浙江,温州,325035;温州医学院环境与公共卫生学院,浙江,温州,325035;温州医学院环境与公共卫生学院,浙江,温州,325035;温州医学院环境与公共卫生学院,浙江,温州,325035;温州医学院环境与公共卫生学院,浙江,温州,325035 |
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| 基金项目： | 温州医学院校科研和教改项目 |
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| 摘 要： | 在pH 3.0的柠檬酸-柠檬酸钠缓冲液中,用溴酚蓝作显色剂,建立了一种快速、简便、灵敏、稳定的测定尿蛋白的分光光度法。该法显色络合物的最大吸收波长为620 nm,线性范围0~4.0 g/L,批内变异系数为0.52%,批间变异系数为1.27%,日间变异系数为1.51%,回收率为98.0%~99.3%,与邻苯三酚红比较有良好相关性,回归方程为y=0.030 0.830x,相关系数为r=0.9798。
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| 关 键 词： | 溴酚蓝 尿蛋白 分光光度法 |
| 文章编号： | 1006-446X（2007）06-0037-03 |
| 收稿时间： | 2007-04-17 |
| 修稿时间： | 2007-04-17 |
A Simple Sensitive Bromophenol Blue Reagent to Determine Uric Protein |
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| FANG Fang,QI Yuhai,ZHANG Feng,HE Yuanyuan,ZHAO Changrong,CAO Jianming. A Simple Sensitive Bromophenol Blue Reagent to Determine Uric Protein[J]. Trace Elements Science, 2007, 14(6): 37-39 |
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| Authors: | FANG Fang QI Yuhai ZHANG Feng HE Yuanyuan ZHAO Changrong CAO Jianming |
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| Abstract: | A simple,sensitive and stable method to determination of protein in urine by spectrophotometry with bromophenol blue(BPB) was established.In the pH 3.0 citric acid buffer system,the maximum absorption of the complex compound was at 620 nm.The linearity was 0~4.0 g/L.The within run,between run and between day CV was respectively 0.52%,1.27% and 1.51%.The recoveries ranged from 98.0% to 99.7%.The correlation between results by this method(y) and pyrogallol red(x) was y=0.030 0.830 x,r=0.979 8. |
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| Keywords: | Bromophenol blue uric protein spectrophotometry |
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