Preparative separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconitum sinomontanum Nakai by high-speed counter-current chromatography |
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| Authors: | Yang Fuquan Ito Yoichiro |
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| Institution: | Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1676, USA. |
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| Abstract: | Analytical high-speed counter-current chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate alkaloids from Aconitum sinomontanum Nakai. The optimum solvent systems CHCl3-MeOH-0.3 M/0.2 M HCl (4:1.5:2, v/v) thus obtained led to the successful separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from 60 to 500 mg of crude alkaloid sample by preparative HSCCC separation. |
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