Disruption of lipid rafts impairs the production of nitric oxide in lipopolysaccharide-stimulated murine RAW264.7 macrophages |
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| Authors: | Shen Zhang Huajun Jin Juefei Zhou Taotao Wei |
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| Institution: | (1) Department of Laboratory Medicine, Huaihua Medical College, Huaihua, 418000, China;(2) Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing, 100101, China;(3) Graduate School of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China |
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| Abstract: | Upon stimulation with lipopolysaccharide, murine RAW264.7 macrophages generated a high amount of nitric oxide. Pretreatment
of macrophages with methyl-β-cyclodextrin, which disrupted the lipid raft microdomains of the plasma membrane, inhibited the generation of nitric oxide
by down-regulating the expression of inducible nitric oxide synthase. Methyl-β-cyclodextrin exposure significantly inhibited the degradation of IκB-α, blocked the translocation of p65/RelA into the nuclei and prevented the activation of the NF-κB signaling pathway. The results suggest that the expression of inducible nitric oxide synthase and the consequent production
of nitric oxide depend on the integrity of the lipid rafts. |
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| Keywords: | RAW 264 7 macrophage nitric oxide lipopolysaccharide iNOS NF-κ B lipid raft methyl-β -cyclodextrin |
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