Forensic analysis of anthraquinone,azo, and metal complex acid dyes from nylon fibers by micro-extraction and capillary electrophoresis |
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Authors: | Amy R Stefan Christopher R Dockery Alexander A Nieuwland Samantha N Roberson Brittany M Baguley James E Hendrix Stephen L Morgan |
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Institution: | (1) Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA;(2) Present address: Polymathic Analytical Labs, 3737 Industrial Blvd, Orangeburg, SC 29118, USA;(3) Present address: Department of Chemistry &; Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA;(4) Present address: Voridian, Hwy. 21 S, Box 1782, Columbia, SC 29202-1782, USA;(5) Present address: Washoe Co. Sheriff Forensic Science Division, 911 Parr Blvd, Reno, NV 89512, USA; |
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Abstract: | The extraction and separation of dyes present on textile fibers offers the possibility of enhanced discrimination between
forensic trace fiber evidence. An automated liquid sample handling workstation was programmed to deliver varying solvent combinations
to acid-dyed nylon samples, and the resulting extracts were analyzed by an ultraviolet/visible microplate reader to evaluate
extraction efficiencies at different experimental conditions. Combinatorial experiments using three-component mixture designs
varied three solvents (water, pyridine, and aqueous ammonia) and were employed at different extraction temperatures for various
extraction durations. The extraction efficiency as a function of the three solvents (pyridine/ammonia/water) was modeled and
used to define optimum conditions for the extraction of three subclasses of acid dyes (anthraquinone, azo, and metal complex)
from nylon fibers. The capillary electrophoresis analysis of acid dye extracts is demonstrated using an electrolyte solution
of 15 mM ammonium acetate in acetonitrile/water (40:60, v/v) at pH 9.3. Excellent separations and discriminating diode array spectra are obtained even for dyes of similar color.
Figure Capillary electropherogram of three acid dyes extracted from nylon 6,6 thread |
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Keywords: | Forensic analysis Nylon fibers Extraction of acid dyes Capillary electrophoresis |
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