Voltammetric determination of dopamine in the presence of ascorbic and uric acids using partial least squares regression: determination of dopamine in human urine and plasma |
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Authors: | Morteza Bahram Khalil Farhadi Farzin Arjmand |
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Institution: | (1) Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran |
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Abstract: | A new differential pulse voltammetric method for dopamine determination at a bare glassy carbon electrode has been developed.
Dopamine, ascorbic acid (AA) and uric acid (UA) usually coexist in physiological samples. Because AA and UA can be oxidized
at potentials close to that of DA it is difficult to determine dopamine electrochemically, although resolution can be achieved
using modified electrodes. Additionally, oxidized dopamine mediates AA oxidation and the electrode surface can be easily fouled
by the AA oxidation product. In this work a chemometrics strategy, partial least squares (PLS) regression, has been applied
to determine dopamine in the presence of AA and UA without electrode modification. The method is based on the electrooxidation
of dopamine at a glassy carbon electrode in pH 7 phosphate buffer. The dopamine calibration curve was linear over the range
of 1–313 μM and the limit of detection was 0.25 μM. The relative standard error (RSE %) was 5.28%. The method has been successfully
applied to the measurement of dopamine in human plasma and urine.
![MediaObjects/11532_2009_57_Fig1_HTML.jpg](/content/h4814x11311p4644/MediaObjects/11532_2009_57_Fig1_HTML.jpg) |
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Keywords: | Partial least squares Differential pulse voltammetry Dopamine |
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