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Continuous Production of Thermostable α-Amylase by Immobilized Bacillus Cells in a Fluidized-Bed Reactor |
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| Authors: | S V Ramakrishna R Jamuna A N Emery |
| Institution: | (1) Fermentation Section, Regional Research Laboratory (CSIR), 695019 Trivandrum, India;(2) Biotechnology Institute 519 Wartik Laboratory, The Pennsylvania State university, 16802 University Park, PA;(3) Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK;(4) Centre for Biochemical Engineering, Department of Chemical Engineering, University of Birmingham, P.O. Box 363, B15 2TT Birmingham, UK |
| Abstract: | The exponential cells ofBacillus cereus immobilized in calcium alginate and spun into microcylindrical particles were used in a fluidized-bed reactor for continuous synthesis of thermostable α-amylase. The reactor was operated over a period of 30 d with a dilution rate of 0.33 h-1, producing 1000–1200 U/mL of enzyme. The productivity of the reactor was in the range of 330–396 kU/h. A 20-fold increase in the productivity with respect to batch fermentation with free cells was attained. |
| Keywords: | Immobilization fluidized bed α -amylase Bacillus cells Ca-alginate |
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