博莱霉素与DNA在镍离子注入修饰电极上的相互作用 及其应用

博莱霉素(BLM)在0.1mol/LHOAc-NaOAc缓冲溶液(pH4.62)中，在Ni/GCE离子注入修饰电极上有一灵敏的还原峰，峰电位为-1.16V(vs.SCE)，峰电流与BLM浓度有关。用线性扫描和循环伏安法研究体系的行为表明，体系为具有加速作用的不可逆过程，是注入的Ni加速BLM的还原。引入DNA后，BLM的峰电位为-1.15V(vs.SCE),与未加入DNA前几乎完全一致；只使峰电流降低，形成一种非电活性的结合物，求得该结合物的结合比为BLM：DNA=3：1，结合常数为β=3.16×10^16，用线性扫描和循环伏安法，并辅以紫外可见光谱法等手段研究表明，电极过程仍为不可逆过程，与未加入DNA时一样。加入DNA后，BLM的峰电流降低，可用于DNA的测定，回收率在96.8～103.9%之间.

Study on the interaction between bleomycin and DNA at Ni/GCE ion implantation modified electrode and its application

With a Ni/GCE on implantation modified electrode in 0.1 mol/L HOAc - NaOAc solution (pH 4.62), a sensitive reduction peak of bleomycin(BLM) was obtained by linear-sweep voltammetry. The peak potential is - 1. 16 V(vs.SCE). The peak current is proportional to the concentration of BLM.The electrochemical behavior of the system was also studied by cyclic voltammetry. The experimental results depict that the electrode process at Ni/GCE is irreversible and the reduction of BLM is accelerated obviously. Measurements of AES show that Ni is surely implanted into the surface of GCE.after adding DNA to the solution containing BLM, the peak potential is almost no change, but the peak current of BLM is decreased. The interaction between BLM and DNA at a Ni/GCE modified electrode was studied by linear sweep and cyclic voltammetry. An inactive complex of BLM with DNA was formed and resulted in a decrease of BLM concentration as well as the reduction current. The composition of the complex is estimated as BLM: DNA=3:1 and the combination constant is calculated as 3.16×10^16. Its electrochemical behavior is the same as that in the absence of DNA. The decrease in the peak current is proportional to DNA concentration and can be used to the detemination of the concentration of DNA.