| 欧鳗“狂游病”病毒的分离及观察 |
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| 引用本文： | 颜青,颜江华,方莹,黄印尧,倪子绵,陈信忠.欧鳗“狂游病”病毒的分离及观察[J].厦门大学学报(自然科学版),2001,40(5):1175-1178. |
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| 作者姓名： | 颜青 颜江华 方莹 黄印尧 倪子绵 陈信忠 |
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| 作者单位： | 1. 厦门大学生命科学学院抗癌研究中心,
2. 厦门大学化学化工学院电镜室, |
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| 摘 要： | 患“狂游病”欧鳗的主要脏器经电镜切片观察表明：该病毒主要存在于病鳗的脑、心脏和肌肉组织中,存在有一大小约为80－100nm的圆形病毒,成熟的病毒颗粒具有清晰可见的包膜结构,病鳗组织匀浆后,经蔗糖分步超速离心法,RNA酶及DNA酶消化去除组织内DNA、RNA后,获得纯化的病毒颗粒。又经蛋白酶K消化后得到病毒的核酸,以DNAaseI或RNAaseA分别消化,经1％的琼脂糖凝胶电泳证实该病毒为DNA病毒。
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| 关 键 词： | 欧鳗 病毒分离 “狂游病”病毒 欧洲鳗鲡 包膜结构 核酸 |
| 文章编号： | 0438-0479(2001)05-1175-04 |
| 修稿时间： | 2000年6月30日 |
Isolation and Examination of "Mad Swimming Symptom"Virus of Anguilla Anguilla |
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| YAN Qing ,YAN Jiang hua ,FANG Ying ,HUANG Yin yao ,NI Zi mian ,CHEN Xin zhong.Isolation and Examination of "Mad Swimming Symptom"Virus of Anguilla Anguilla[J].Journal of Xiamen University(Natural Science),2001,40(5):1175-1178. |
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| Authors: | YAN Qing YAN Jiang hua FANG Ying HUANG Yin yao NI Zi mian CHEN Xin zhong |
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| Institution: | YAN Qing 1,YAN Jiang hua 1,FANG Ying 1,HUANG Yin yao 2,NI Zi mian 3,CHEN Xin zhong 2 |
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| Abstract: | Ultrastructure of tissues of Anguilla anguilla with " ma d swimming symptom" showed that a round virus of 80 100 nm in diameter, contai ning capsule, ex is ted in brain, heart and muscle tissues of sick Anguilla anguilla . From its hom ogenized heart or muscle tissues whose nucleic acid was removed with DNAase and RNAase, the virus was purified with sucrose density gradient supercentrifugation assay. The purified virus was demonstrated to be a DNA virus through nucleic ac id extraction and nuclease test. |
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| Keywords: | Anguilla anguilla Virus Isolation |
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