Protein tango: the toolbox to capture interacting partners |
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Authors: | Rutkowska Anna Schultz Carsten |
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Affiliation: | Cell Biology & Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstr. 1, 69117 Heidelberg (Germany). |
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Abstract: | The evaluation of protein function in the context of the whole cell is crucial for understanding of living systems. In this context, the identification and modulation of protein-protein interactions in and outside cells is of ample importance. Several methods have been developed in the past years to detect and/or actively induce protein-protein interactions in living cells. As a result, tools are now available to manipulate intracellular events by reversible or irreversible cross-linking of proteins in a specific manner. These techniques open many new doors and enable the dissection of complicated protein networks. Herein we describe which cross-linkers and inducers of dimerization are out there and how to make use of this great toolbox. |
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Keywords: | cross‐linkers dimerizers protein–protein interactions small molecules |
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