Multilayer Microcapsules for FRET Analysis and Two‐Photon‐Activated Photodynamic Therapy |
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| Authors: | Dr. Yang Yang Huiling Liu Prof. Dr. Mingjuan Han Bingbing Sun Prof. Dr. Junbai Li |
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| Affiliation: | 1. CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, China;2. College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, China;3. Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China |
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| Abstract: | Microcapsules obtained by layer‐by‐layer assembly provide a good platform for biological analysis owing to their component diversity, multiple binding sites, and controllable wall thickness. Herein, different assembly species were obtained from two‐photon dyes and traditional photosensitizers, and further assembled into microcapsules. Fluorescence resonance energy transfer (FRET) was shown to occur between the two‐photon dyes and photosensitizers. Confocal laser scanning microscopy (CLSM) with one‐ and two‐photon lasers, fluorescence lifetime imaging microscopy (FLIM), and time‐resolved fluorescence spectroscopy were used to analyze the FRET effects in the microcapsules. The FRET efficiency could easily be controlled through changing the assembly sequence. Furthermore, the capsules are phototoxic upon one‐ or two‐photon excitation. These materials are thus expected to be applicable in two‐photon‐activated photodynamic therapy for deep‐tissue treatment. |
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| Keywords: | dyes FRET microcapsules photodynamic therapy photosensitizers |
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