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N‐Linked Glycosyl Auxiliary‐Mediated Native Chemical Ligation on Aspartic Acid: Application towards N‐Glycopeptide Synthesis |
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| Authors: | Hua Chai Dr. Kim Le Mai Hoang Minh Duy Vu Dr. Kalyan Pasunooti Prof. Dr. Chuan‐Fa Liu Prof. Dr. Xue‐Wei Liu |
| Affiliation: | 1. School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore;2. School of Biological Sciences, Nanyang Technological University, Singapore, Singapore |
| Abstract: | A practical approach towards N‐glycopeptide synthesis using an auxiliary‐mediated dual native chemical ligation (NCL) has been developed. The first NCL connects an N‐linked glycosyl auxiliary to the thioester side chain of an N‐terminal aspartate oligopeptide. This intermediate undergoes a second NCL with a C‐terminal thioester oligopeptide. Mild cleavage provides the desired N‐glycopeptide. |
| Keywords: | aspartic acid auxiliary mediation CTLA-4 dual native chemical ligation N-glycopeptide synthesis |