Determination of carbaryl and its metabolite 1-naphthol in commercial formulations and biological fluids

A simple and sensitive method for the determination of carbaryl in whole blood and commercial formulations, based on normal, and synchronous first- and second-derivative fluorescence spectra, is presented. Solvent effects on the spectral characteristics of carbaryl solutions and the influences of instrumental parameters are described in detail. Two methods have been developed, with neutral (for carbaryl) and basic (for 1-naphthol) media. Detection limits of 0.9 and 0.7 ng/ml were achieved for carbaryl and 1-naphthol, respectively, with the first-derivative approach.