Universal on-line detector for high-performance liquid chromatography via magneto-optical rotation |
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| Authors: | Kawazumi H Nishimura H Otsubo Y Ogawa T |
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| Affiliation: | Department of Molecular Science and Technology, Kyushu University, Kasuga 816, Japan. |
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| Abstract: | Detecting changes in magneto-optical rotation is useful as a universal on-line detector for high-performance liquid chromatography. Such apparatus is similar to a polarimeter except for the external magnetic field on a flow cell. Two modulation modes suitable for the magneto-optical rotation detector are discussed. Use of a semiconductor laser provides better sensitivity than a He-Ne laser. The detection limit is 0.006% (w/w) for polyethylene glycol 20000 in a 20-mul injection. |
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