| 光谱法对照研究肌红蛋白及其突变体Mb(D60K)与H_2O_2的相互作用 |
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| 作者姓名： | Zhi QY Tang Q Cao HY An LM Zhang YY Zheng XF |
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| 作者单位： | 1. 大连大学,辽宁省生物有机化学重点实验室,辽宁大连116622;大连大学生物工程学院,辽宁大连116622
2. 大连大学生物工程学院,辽宁大连,116622 |
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| 基金项目： | 国家自然科学基金项目(20871024); 辽宁省优秀人才培养计划项目(RC-04-10); 辽宁省高校创新团队项目(2006T002,2008T005,2009T003); 辽宁省教育厅项目(2009A069); 大连市科技计划项目(2008E11SF170)资助 |
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| 摘 要： | 为了解肌红蛋白(Mb)表面60位天冬氨酸(Asp)突变为赖氨酸(Lys)后对蛋白结构稳定性的影响,本文通过紫外-可见吸收光谱、荧光光谱和停流荧光光谱对照研究了模拟生理条件下野生型肌红蛋白Mb(WT)及其突变体Mb(D60K)与过氧化氢(H2O2)的相互作用。结果表明:在Mb(D60K)与H2O2发生相互作用过程中,铁卟啉部位的紫外和荧光发射光谱数据与Mb(WT)相比,性质与功能均表现出显著差异。虽然只有一个氨基酸的改变,但其结构和性质发生明显变化,说明60位氨基酸在稳定蛋白结构中有重要的作用。同步荧光光谱和停流光谱的结果同样表明Mb(D60K)的结构与功能受H2O2的影响较小,Mb(WT)受H2O2影响明显。综合分析表明,Mb(D60K)在与H2O2相互作用过程中,蛋白结构稳定性提高。
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| 关 键 词： | 肌红蛋白 Mb(D60K) 过氧化氢 光谱法 蛋白结构 |
Comparison between myoglobin and its mutant(D60K) interacting with hydrogen peroxide by spectrum |
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| Zhi QY,Tang Q,Cao HY,An LM,Zhang YY,Zheng XF.Comparison between myoglobin and its mutant(D60K) interacting with hydrogen peroxide by spectrum[J].Spectroscopy and Spectral Analysis,2011,31(9):2512-2516. |
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| Authors: | Zhi Qiu-Yan Tang Qian Cao Hong-Yu An Liang-Mei Zhang Ying-Ying Zheng Xue-Fang |
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| Institution: | Liaoning Key Lab of Bioorganic Chemistry, Dalian University, Dalian 116622, China. dlzhiqy@163.com |
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| Abstract: | To characterize the roles played by surface-charged residue Asp60 in the structure stability of myoglobin when it was replaced with Lys,the interaction of myoglobinMb(WT)] and its mutantMb(D60K)] with hydrogen peroxide(H2O2) were studied by the method of ultraviolet-visible(UV-Vis) absorption spectroscopy,fluorescence spectroscopy and stopped-flow fluorescence spectroscopy under simulative physiological conditions.There are remarkable differences between Mb(D60K) and Mb(WT) in the UV-Vis absorption spectr... |
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| Keywords: | Myoglobin Mb(D60K) Hydrogen peroxide Spectroscopy Protein structure |
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