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| 铁蛋白脱铁机制的建立及脱铁前后光谱变化的研究 | |
| 作者姓名： | Liu WY Zhang J Tong JM Zhao GH |
| 作者单位： | 1. 石河子大学食品学院,新疆石河子 832003;国家蛋品工程技术研究中心,北京102115 2. 石河子大学食品学院,新疆石河子 832003;中国农业大学食品科学与营养工程学院,北京100083 3. 石河子大学食品学院,新疆石河子,832003 4. 中国农业大学食品科学与营养工程学院,北京,100083 |
| 基金项目： | 国家自然科学基金项目(30972045)资助 |
| 摘 要： | 建立了一套可以实现铁蛋白脱铁的机制并对脱铁前后铁蛋白的光谱进行了分析。应用连二亚硫酸钠(Sodium Hyposulfite)为还原剂与铁蛋白发生还原反应,促使铁蛋白矿化铁的还原释放,此过程中使用2,2-联吡啶(2,2-Dipyridyl)实时监测脱铁状况。通过ICP-MS检测显示,脱铁铁蛋白制备成功。紫外分析结果表明铁蛋白在脱铁之前没有吸收峰,而在脱铁后在280 nm处具有明显的吸收峰。荧光分析结果表明,铁蛋白脱铁前没有荧光发射光谱,而在脱铁后则具有荧光发射光谱。 |
| 关 键 词： | 铁蛋白 脱铁铁蛋白 ICP-MS 荧光光谱 紫外光谱 |
Study on of deferrization mechanism of apoferritin and the effect of spectra variation with holoferritin |
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| Liu WY,Zhang J,Tong JM,Zhao GH.Study on of deferrization mechanism of apoferritin and the effect of spectra variation with holoferritin[J].Spectroscopy and Spectral Analysis,2011,31(9):2552-2554. | |
| Authors: | Liu Wen-Ying Zhang Jian Tong Jun-Mao Zhao Guang-Hua |
| Institution: | College of Food, Shihezi University, Shihezi 832003, China. skyocean_2004@163.com |
| Abstract: | The deferrization mechanism of apoferritin was established,and the spectra variation of apoferritin was compared with that of holoferritin.Sodium hyposulfite is a strong reducing agent,therefore,was applied to deoxidize holoferritin to release iron ion,and connection of iron of buffer was measured by the 2,2-dipyridyl.Apoferritin was detected by ICP-MS.Holoferritin was found to have no absorption compared with apoferritin by UV analysis, and have no fluorescence emission spectra in contrast with apoferritin... |
| Keywords: | Holoferritin Apoferritin Spectra ICP-MS |
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