Evaluation of ferritin-overexpressing brain in newly developed transgenic mice |
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Authors: | Hasegawa Sumitaka Saito Shigeyoshi Takanashi Jun-ichi Morokoshi Yukie Furukawa Takako Saga Tsuneo Aoki Ichio |
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Affiliation: | a Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japanb Graduate School of Medicine, Tohoku University, Sendai 980-8575, Japanc Department of Pediatrics, Kameda Medical Center, Kamogawa 296-8602, Japan |
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Abstract: | Aberrant expression of ferritin, a major iron-binding protein, has shown to be involved in neurodegenerative diseases. In this study, we generated transgenic (Tg) mice of human ferritin heavy chain (FTH) gene and investigated the effects of ferritin overexpression in FTH-Tg brain by 1H-MRI and 1H-MRS. The mice displayed no apparent neurological symptoms, and no specific morphological and T2 alterations were found in the brain by MRI, and not even by histological studies. 1H-MRS, however, revealed that some metabolic markers were significantly altered in FTH-Tg brains compared to wild-type control brains, such as decreases in myo-inositol and glutamine, and an increase in lactate. Our present studies suggested that despite the absence of neurological, morphological, T2, and histological signatures, brain metabolisms were significantly affected in FTH-Tg mice. This study also highlights the usefulness of 1H-MRS in the analysis of transgenic mouse models. |
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Keywords: | 1H-MRS Ferritin Iron Transgenic mouse |
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