New strategy for the identification of amino acids by TLE and TLC using basic or buffered mobile phases |
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Authors: | R L Munier S Meunier |
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Institution: | (1) Institut Pasteur, C.N.R.S., 28 Rue du Docteur-Roux, F-75015 Paris, France |
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Abstract: | Summary Direct, simple and reliable means for characterization of the common amino acids, using mixtures of structural analogues of amino acids and combinations of low-voltage electrophoresis (1.04 mol dm–3 formic acid) and chromatography (tert-butanol/methanol/pyridine/formic acid/water, 33:43:9.6:0.4:20, v/v, methanol/pyridine/formic acid/water, 70:9.6:0.4:20, v/v, or tertpentanol/methylethylketone/pyridine/water, 37.5:37.5:5:20, v/v) on amorphous cellulose thin-layer are given. The efficiency of the procedures is evaluated in various examples.Abbreviations used: TLC = thin-layer chromatography, TLE = thin-layer electrophoresis, TLP = thin-layer plate; other abbreviations: see Table I and end of text.for structural analogues of amino acids and glucosamine, see Table I. |
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Keywords: | Identification of amino acids Thin-layer chromatography Thin-layer electrophoresis |
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