基于红外ATR光谱的组织液中葡萄糖信息检测方法研究

通过测量组织液中的葡萄糖浓度获得血糖信息是目前实现血糖连续检测的主要方法，中红外ATR光谱法在分析葡萄糖等生物小分子成分信息方面具有显著优势，但是如何利用中红外ATR光谱法检测皮下组织液中的葡萄糖信息仍是尚未解决的问题。在对中红外穿透深度理论分析的基础上开展了自然状态和渗透状态下人体皮下组织液葡萄糖的中红外ATR光谱检测实验。首先探究将中红外光直接入射到人体皮肤表层上，采集自然状态下人体手指皮下组织液葡萄糖的ATR光谱数据，讨论中红外直接穿透皮下组织获得葡萄糖信息的可行性;在此基础上，通过采用低频超声和真空负压的方法促进组织液渗透到皮肤表层，采集其中红外ATR光谱进而分析和判断是否含有葡萄糖特异信息。由于二维相关光谱技术在红外光谱等光谱研究领域分析分子间反应和物质的吸收峰信息来源时有很高的分辨率和很好的适用性，利用二维相关光谱技术分别分析皮肤表层在自然状态和渗透状态的实验条件皮下组织体中葡萄糖信息。实验结果表明：中红外ATR光谱法无法直接应用于皮下组织液的葡萄糖浓度检测，利用物理或化学的方法促进组织液渗透到皮肤表层，是实现中红外ATR光谱法检测皮下组织液中葡萄糖的一个发展方向。

Study on Detection Methods of Interstitial Fluid Glucose Concentration Based on Infrared Attenuated Total Reflection

Measuring the glucose concentrations in the interstitial fluid is currently the main method to achieve the continuous blood glucose monitoring. The MIR-ATR(Mid-infrared，Attenuated Total Reflection)Spectroscopy has prominent advantage on the analysis of small biological molecule for composition information like the glucose, but it is still an unresolved problem that how to detect the subcutaneous glucose concentration by using the MIR-ATR Spectroscopy. In the present paper, we carry out the experiment based on MIR-ATR for the detection of subcutaneous glucose information on both the natural state and the penetration state based on the theoryanalysis of MIR penetration depth. Firstly, collect spectral data of the subcutaneous glucose concentration of human finger on the natural state were collected as the light shined the skin directly, and it was discussed whether the MIR can penetrate the skin to get the information of subcutaneous glucose. On this basis, collect spectral data of the subcutaneous glucose concentration of human finger at the penetration state were collected when the Interstitial fluid is permeated to the surface layer by using low-frequency ultrasound and vacuum, then it analyzed that whether it can detect the glucose-specific information or not. As the two-dimensional correlation spectroscopy has high resolution and good versatility, it is widely used to analyze the inter-molecular reaction and judge the absorption peaks information in many fields including the MIR spectroscopy field, so we choose the Two-dimensional correlation spectroscopy to analyze the information of subcutaneous glucose concentration at the natural state and the penetration state. The experiment result shows that the MIR-ATR spectroscopy can’t be applied in the detection of subcutaneous glucose concentrationdirectly, and it is a promising direction to make the Interstitial fluid permeated to the surface layer by the physical methods or chemical methods.