Study
on interaction between antibiotics and Escherichia
coli DH5α by microcalorimetric method |
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| Authors: | L N Yang F Xu L X Sun Z C Tan H D Tan Z B Zhao J G Liang |
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| Institution: | (1) Materials and Thermochemistry Laboratory, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P.R. China, 116023, 457 Zhongshan Road;(2) Graduate School of the Chinese Academy of Sciences, Beijing, P.R. China, 100039;(3) Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P.R. China, 116023, 457 Zhongshan Road;(4) Hunan Institute of Drug Detection, Changsha, P.R. China, 410001 |
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| Abstract: | A microcalorimetric
technique based on the bacterial heat output was applied to evaluate the influence
of antibiotics PIP (Piperacillin Sodium)
and composite preparation of PIP and SBT (Sulbactam
Sodium) on the growth of E. coli
DH5α. The power–time curves of the growth metabolism of E. coli DH5α were studied using a TAM Air Isothermal
Microcalorimeter at 37°C. By analyzing the power–time curves, the
parameters such as growth rate constants (k),
inhibitory ratio (I), the maximum heat
power (P
m) and the
time of the maximum heat power (t
m)
were obtained. The results show that different concentrations of antibiotics
affect the growth metabolism of E. coli
DH5α. The PIP in the concentration range of 0–0.05 μg mL–1
has a stimulatory effect on the E. coli
DH5α growth, while the PIP of higher concentrations (0.05 –0.25
μg mL–1) can inhibit its growth. It seems
that the composite preparation composed of PIP and SBT cannot improve the
inhibitory effect on E. coli DH5α
as compared with the PIP. |
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| Keywords: | E coli DH5α inhibition microcalorimetry Piperacillin Sodium Sulbactam Sodium |
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