| 米曲霉固态发酵产淀粉酶条件优化的研究 |
| |
| 引用本文： | 卢福芝,钱丰,韦江露,屈桂群,李欣.米曲霉固态发酵产淀粉酶条件优化的研究[J].河池师专学报,2013(5):14-18. |
| |
| 作者姓名： | 卢福芝 钱丰 韦江露 屈桂群 李欣 |
| |
| 作者单位： | [1]河池学院化学与生物工程学院,广西宜州546300 [2]广西广维化工有限责任公司,广西宜州546307 |
| |
| 基金项目： | 河池学院青年科研基金资助课题（2011A-N004）. |
| |
| 摘 要： | 以米曲霉A—l为出发菌株，在研究培养基加水量、温度、无机盐和培养时间等单因素的基础上，采用正交试验方法进行固态发酵产淀粉酶条件优化研究，并对正交试验结果进行了验证。试验结果表明，该菌株固态发酵产淀粉酶的最优条件为：加水量10mL，麸皮与豆饼粉的比例为8：2，MgS047H：0添加量为1％，在30℃发酵84h，酶活达269U／g。
|
| 关 键 词： | 米曲霉 固态发酵 淀粉酶 |
A Study on Solid - state Fermentation Conditions of the Amylase from Aspergillus Oryzae |
| |
| LU Fu -zhi,QIN Feng,WEI Jiang -lul,QU Gui -qun,LI Xin.A Study on Solid - state Fermentation Conditions of the Amylase from Aspergillus Oryzae[J].Journal of Hechi Normal College,2013(5):14-18. |
| |
| Authors: | LU Fu -zhi QIN Feng WEI Jiang -lul QU Gui -qun LI Xin |
| |
| Institution: | 1 ( 1. School of Chemistry and Biological Engineering, Hechi University, Yizhou, Guangxi 546300 ; 2. Guangxi Guangwei Chemical Co., Ltd, Yizhou, Guangxi 546307, China) |
| |
| Abstract: | Taking Aspergillus oryzae A - 1 as the original strain, based on the study of the elements of wa- ter addition, inorganic salt and incubation time, orthogonal experimental design has been used to study the cultiva- ting conditions of the production of amylase from Aspergillus. And the results have been verified. The results show that the optimum condition is ： the additive quantity of water is 10 ml ; the proportion of bran to that of soybean cake powder is 8： 2; the additive quantity of MgSO47H20 is 1% ; the temperature of 84 h fermentation is 30 C. Under this condition, the activity of amylase can reach 269 U/g. |
| |
| Keywords: | Aspergillus oryzae solidfermentation amylase |
| 本文献已被 维普 等数据库收录！ |
|
