| Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifolium |
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| 作者姓名： | REN Jie LIU Yan-qiu YANG Li NI Rui YOU Song |
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| 作者单位： | REN Jie，LIU Yan-qiu，YANG Li，NI Rui and YOU Song *School of Pharmaceutical Engineering，Shenyang Pharmaceutical University，Shenyang 110016，P. R. China |
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| 摘 要： | Crepidiastrum sonchifolium(Bunge),whose activeingredients are sesquiterpenes,triterpene,flavone,andlignanoid,has been used as a folk medicine in Asiancountries because of its digestive,diuretic,and anti-in-flammatory activities1,2].It is interesting to k…
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| 关 键 词： | 分子克隆 细菌表达 大根香叶烯A合酶 cDNA 中药 有效成分 |
| 文章编号： | 1005-9040（2006）-05-606-06 |
| 收稿时间： | 2006-04-20 |
Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifolium |
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| REN Jie LIU Yan-qiu YANG Li NI Rui YOU Song.Molecular Cloning and Bacterial Expression of Germacrene A Synthase cDNA from Crepidiastrum sonchifolium[J].Chemical Research in Chinese University,2006,22(5):606-611. |
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| Authors: | Jie REN Yan-qiu LIU Li YANG Rui NI Song YOU |
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| Institution: | aSchool of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, P. R. China |
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| Abstract: | Germacrene A synthase(GAS) catalyzes the biosynthesis of germacrene A, which is a key precursor for sesqui- terpene lactones. Cloning of a novel full-length cDNA encoding GAS from the medicinal plant Crepidiastrum sonchifolium(designated CsGAS) is reported in this study. The cDNA is 1837 bp long and contains a 1680-bp open reading frame encoding a 559 amino-acid protein. The functional expression of the cDNA in Escherichia coli, as an N-terminal thioredoxin fusion protein, with the pET32a vector yielding a recombinant enzyme. Sequence analysis was used to compare this enzyme with the mechanistically related epi-aristolochene synthase from tobacco, and the effect of possible involvement of a number of amino acids in sesquiterpene synthase on product specificity was also discussed. |
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| Keywords: | Germacrene A synthase Crepidiastrum sonchifolium Sesquiterpene synthase Expression Fusion protein |
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