1. Humboldt Universit?t zu Berlin, Unter den Linden 6, 10117, Berlin, Germany 2. In-Process-Control, Schering AG, Müllerstr. 170-178, 13342, Berlin, Germany
Abstract:
Summary This paper demonstrates the possibilities in transfering HPLC methods from porous to non-porous stationary phases. The procedures
are transferred from 125 or 250 mm columns packed with porous stationary phases to 33 mm columns packed with non-porous particles.
It is demonstrated that fast HPLC using non-porous columns reduces analysis times by a factor four to eight. Precision is
comparable to HPLC with porous stationary phases. The costs for HPLC with porous and with nonporous packing materials are
similar. The implementation of these fast HPLC columns is easy because standard equipment can be used.