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Characterization of immobilized nuclease P1 |
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| Authors: | Quo-Qing Ying Lu-E Shi Xiao-Ying Zhang Wei Chen Yu Yi |
| Affiliation: | (1) College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China;(2) College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China;(3) College of Pharmacy, South Dakota State University, 57007 Brookings, SD |
| Abstract: | To improve the efficiency of the use of nuclease P1, enzyme immobilization technology was applied using nuclease P1. Characterization of immobilized nuclease P1 on different supports was studied. The results showed that the optimum pH and temperature of nuclease P1 immobilized via different supports were enhanced. The immobilized enzyme was obviously stable when stored for long periods and was reusable. The best results were obtained when nuclease P1 was immobilized on chitosan nanoparticles. The nanoparticles were applied to protect the activity of nuclease P1 and improved enzyme activity by 13.17% over that of free nuclease P1 at the same conditions. The Michaelis constant Km and V max were determined for free and immobilized enzyme as well. |
| Keywords: | Chitosan nanoparticles cellulose nuclease P1 immobilization |
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