| 激光诱导血红蛋白分子的多光子荧光 |
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| 引用本文： | 张晶如,徐英武,邓玉妹,吴存恺,江寿平,连少辉. 激光诱导血红蛋白分子的多光子荧光[J]. 光学学报, 1987, 0(10) |
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| 作者姓名： | 张晶如 徐英武 邓玉妹 吴存恺 江寿平 连少辉 |
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| 作者单位： | 中国科学院安徽光学精密机械研究所(张晶如，徐英武，邓玉妹，吴存恺)，中国科学院上海生物化学研究所(江寿平)，中国科学院上海生物化学研究所(连少辉) |
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| 摘 要： | 本文首次报道了血红蛋白吸收二个和四个1.06μm的光子后所发射的荧光特性,并分析了其产生的原因。
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| 关 键 词： | 生物分子光谱 多光子荧光 血卟啉衍生物 血红蛋白 |
Laser-induced multiphoton fluorescence of hemoglobin |
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| ZHANG JINGRU,XU YINGWU,DENG YUMEI AND WU CUNKAI. Laser-induced multiphoton fluorescence of hemoglobin[J]. Acta Optica Sinica, 1987, 0(10) |
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| Authors: | ZHANG JINGRU XU YINGWU DENG YUMEI AND WU CUNKAI |
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| Abstract: | Hemoglobin and other samples were irradiated by the 1064 nm light of a nanosecond pulse Nd:YAG laser. At room temperature, we first observed two red fluorescence bands which result from absorbing two 1064 nm photons in hemoglobin (Hb), and we measured the fluorescence emission spectra at red region for Hb and hemotoporphyrin derivative (HpD). Both Hb and HpD have two fluorescence emission maxima. A red shift in wavelength and a mirror image in the intensity of fluorscence emissions by two-photon excitation relative to that by one-photon (532-nm) excitation were observed. The experimental analysis showed that the fluorescence of Hb originates from the porphyrin ring of the heme group, just as that of HpD from the porphyrin ring. Besides, we also observed the fluorescence emission at UV 392-nm for Hb, which may come from the tryptophans in Hb. |
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| Keywords: | biomolecular spectroscopy multiple photon fluorescence hematoporphyrin derivative hemoglobin. |
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