Direct Bioelectrocatalysis by NADP‐Reducing Hydrogenase from Pyrococcus furiosus |
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| Authors: | Oleg G Voronin Daan J van Haaster Elena E Karyakina Wilfred R Hagen Arkady A Karyakin |
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| Institution: | 1. Chemistry Faculty of M.?V. Lomonosov Moscow State University, 119899, Moscow, Russia;2. Department of Biotechnology, Delft University of Technology, The Netherlands |
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| Abstract: | The direct bioelectrocatalysis by an NAD(P)‐reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis 1–4], which were in fact unsuccessful, in our report an effective electrocatalysis by Pyrococcus furiosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm?2 at 100 mV overpotential and at 75 °C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)‐independent hydrogenases. |
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| Keywords: | Bioelectrocatalysis Hydrogen equilibrium potential Hydrogenase NAD(P) Enzyme electrode |
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