Amperometric Biosensor for Hydrogen Peroxide,Using Supramolecularly Immobilized Horseradish Peroxidase on the β‐Cyclodextrin‐Coated Gold Electrode |
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| Authors: | Conrado Camacho Juan?C Matías Belkis Chico Roberto Cao Leissy Gómez Benjamin?K Simpson Reynaldo Villalonga |
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| Institution: | 1. Center for Enzyme Technology, University of Matanzas, Autopista a Varadero km 3 1/2, Matanzas 44740, Cuba;2. Laboratory of Bioinorganic Chemistry, Faculty of Chemistry, University of Havana, Havana 10400, Cuba;3. Food Science and Agricultural Chemistry Department, McGill University (Macdonald Campus), 21,111 Lakeshore Road, Ste Anne de Bellevue, Québec, Canada H9X 3V9 |
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| Abstract: | Horseradish peroxidase, previously modified with 1‐adamantane moieties, was supramolecularly immobilized on gold electrodes coated with perthiolated β‐cyclodextrin. The functionalized electrode was employed for the construction of an amperometric biosensor device for hydrogen peroxide using 1 mM hydroquinone as electrochemical mediator. The biosensor exhibited a fast amperometric response (6 s) and a good linear response toward H2O2 concentration between 12 μM and 450 μM. The biosensor showed a sensitivity of 1.02 mA/M cm2, and a very low detection limit of 5 μM. The electrode retained 97% of its initial electrocatalytic activity after 30 days of storage at 4 0C in 50 mM sodium phosphate buffer, pH 7.0. |
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| Keywords: | Biosensor for hydrogen peroxide β ‐Cyclodextrin Enzyme immobilization Horseradish peroxidase Supramolecular complex |
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