Detection of NADH and Ethanol at Titanium Containing MCM‐41 with Low Overpotential |
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| Authors: | Zhihui Dai Guofei Lu Jianchun Bao Xiaohua Huang Huangxian Ju |
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| Institution: | 1. Department of Chemistry, Nanjing Normal University, Nanjing, 210097, P.?R. China;2. Department of Chemistry, Nanjing Xiaozhuang College, Nanjing, 210017, P.?R. China;3. Department of Chemistry, Nanjing University, Nanjing, 210093, P.?R. China |
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| Abstract: | Titanium‐containing MCM‐41 (Ti‐MCM‐41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of β‐Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the overvoltage of NADH oxidation reaction is observed at 0.28 V vs. SCE. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this material. The proposed sensor shows a highly sensitivity, an acceptable reproducibility and a good stability. The linear range of ethanol is 25–1000 μM and the detection limit is 8.0 μM. Ti‐MCM‐41 modified electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix for the construction of dehydrogenases sensor. |
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| Keywords: | NADH Ti‐MCM‐41 Electrocatalytic Sensors ADH Ethanol |
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