Synthesis and Hybridization Properties of Oligodeoxynucleotides with Long‐Chain Linkers |
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| Authors: | Kohji Seio Takeshi Terada Masahiro Mizuta Akihiro Ohkubo Haruhiko Taguchi Mitsuo Sekine |
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| Institution: | 1. Frontier Collaborative Research Center, Tokyo Institute of Technology;2. CREST, Japan Science and Technology Agency (JST);3. Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta‐cho, Midori‐ku, Yokohama, Japan (phone: +81‐45‐924‐5706;4. fax: +81‐45‐924‐5772) |
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| Abstract: | New oligonucleotides with a long‐chain linker (6,9‐dioxa‐3,12‐diazatetradecane‐1,14‐diyl) in their backbone were synthesized, and their hybridization properties were studied by measurement of their Tm curves and fluorescence spectra. The Tm analyses revealed that these oligonucleotides could bind to their complementary strands despite the presence of the long‐chain linker. We also demonstrated interesting fluorescence properties of oligodeoxynucleotides with an anthracen‐9‐ylmethyl group on one of the two N‐atoms in the long‐chain linker. The fluorescence intensity of these oligonucleotides increased upon their hybridization to the complementary strands and was sensitive to the presence of the mismatch base pairs at a specific position. |
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| Keywords: | Nucleotides Oligodeoxynucleotides Hybridization |
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