Fabrication of Active Horseradish Peroxidase Micropatterns with a High Resolution by Scanning Electrochemical Microscopy |
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| Authors: | Xuemei Li Qiaohong Geng Yingying Wang Zhikun Si Wei Jiang Xiaoli Zhang Wenrui Jin |
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| Affiliation: | School of Chemistry and Chemical Engineering, Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan, 250100, P.?R. China |
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| Abstract: | We used a new reactive species OH? to fabricate active horseradish peroxidase (HRP) micropatterns with a high resolution by scanning electrochemical microscopy (SECM) coupled with a carbon fiber disk electrode as the SECM tip. In this method, except for active HRP micropatterns predesigned other regions on a HRP‐immobilized substrate were deactivated by OH? generated at the tip held at ?1.7 V in 1.0 mol/L KCl containing 2.0×10?3 mol/L benzoquinone (BQ) (pH 8.0). The feedback mode of SECM with a tip potential of ?0.2 V was used to characterize the active HRP micropatterns in 1.0 mol/L KCl containing 2.0×10?3 mol/L BQ and 2.0×10?3 mol/L H2O2. |
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| Keywords: | Microfabrication Biomolecule pattern Enzyme activity Scanning electrochemical microscopy |
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