Studies on the binding of vinpocetine to human serum albumin by molecular spectroscopy and modeling |
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Authors: | Hua Jiang Rong Rong Chen Hong Cui Wang Han Lin Pu Bio-engineering Institute |
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Affiliation: | Bio-engineering Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
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Abstract: | The interaction between vinpocetine(VPC) and human serum albumin(HSA) in physiological buffer(pH 7.40) was investigated by fluorescence,FT-IR,UV-vis absorption and molecular modeling.VPC effectively quenched the intrinsic fluorescence of HSA via static quenching.The binding site number n and apparent binding constant K_a,corresponding thermodynamic parametersΔG,ΔH andΔS at different temperatures were calculated.The synchronous fluorescence and FT-IR spectra were used to investigate the structural change of HSA molecules with addition of VPC.Molecular modeling indicated that VPC could bind to the site I of HSA and hydrophobic interaction was the major acting force,which was in agreement with the binding mode study. |
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Keywords: | Vinpocetine Human serum albumin Fluorescence quenching Molecular modeling |
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