Novel electron transfer mediators based on dichloroindophenol derivatives for lactate oxidase |
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Authors: | Keisuke Hirano Hitoshi Yamato Kazuhiko Kunimoto Masaki Ohwa |
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Abstract: | Novel indophenol derivatives were synthesized and characterized electrochemically with respect to their abilities to act as electron-transfer mediators for lactate oxidase. These compounds showed suitable redox potentials and high second-order rate constants, kmed, in solution for electron-transfer from the reduced enzyme. A lactate sensor using these derivatives demonstrated high sensitivity and good substrate selectivity. This sensor could also achieve excellent durability which retained more than 70% residual activity and good linearity in the range from 0 to 16 mM lactate concentration even after 10 days. |
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Keywords: | Indophenol derivatives Mediator Lactate oxidase Lactate sensor |
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